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cyclin dependent kinase inhibitor 1a  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology cyclin dependent kinase inhibitor 1a
    Cyclin Dependent Kinase Inhibitor 1a, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 8467 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cyclin+dependent+kinase+inhibitor+1a/p21+Antibody/pm41839900-137-51-56
    Average 96 stars, based on 8467 article reviews
    cyclin dependent kinase inhibitor 1a - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: Targeted Deletion of Capn4 in Cells of the Chondrocyte Lineage Impairs Chondrocyte Proliferation and Differentiation
    Article Snippet: .. Western blot analysis was performed as described previously with antibodies against the following proteins: cyclin D1, activating transcription factor 2 (ATF2), phospho-ATF2 (Cell Signaling Technology Inc., Danvers, MA), cyclin D2, cyclin E, cyclin-dependent kinase 2 (cdk2), cdk4, cyclin-dependent kinase inhibitor 1A (p21 Cip1 ), cyclin-dependent kinase inhibitor 1B (p27 Kip1 ), cyclin-dependent kinase inhibitor 1C (p57 Kip2 ) (Santa Cruz), phospho-retinoblastoma protein (threonine 821) (Invitrogen), phospho-p27 Kip1 (serine 10), and phospho-p27 Kip1 (T187) (Abcam Inc., Cambridge, MA) ( 1 ). .. In some experiments, blots were incubated in Restore Western blot stripping buffer (Thermo Scientific, Rockford, IL) and reprobed with other primary antibodies.

    Immunohistochemistry:

    Article Title: Sexual dimorphism in liver cell cycle and senescence signaling pathways in young and old rats
    Article Snippet: .. Primary antibodies used for IHC analysis were: for cell cycle, anti-cyclin-dependent kinase 2 (CDK2) 1:50 (mouse monoclonal SC-6248, Santa Cruz Biotechnology, Dallas, TX, USA), cyclin-dependent kinase 4 (CDK4) 1:400 (mouse monoclonal SC-23896, Santa Cruz), cyclin D1 (CycD, CCND1) 1:50 (mouse monoclonal SC-8396, Santa Cruz), anti-proliferating cell nuclear antigen (PCNA) 1:50 (mouse monoclonal SC-56, Santa Cruz), cyclin-dependent kinase inhibitor 1A (CDKN1A, p21) 1:50 (mouse monoclonal SC-6246, Santa Cruz) and cyclin-dependent kinase inhibitor 2B (CDKN2B, p15) 1:50 (mouse monoclonal SC-377412, Santa Cruz); for cellular senescence, anti-calmodulin 2 (CALM2) 1:50 (mouse monoclonal SC-137079, Santa Cruz), anti-insulin like growth factor binding protein 3 (IGFBP3)1:50 (mouse monoclonal SC-365936, Santa Cruz), anti-mechanistic target of rapamycin (mTOR) 1:50 (mouse monoclonal SC-517464, Santa Cruz), sequestosome 1 (SQSTM1, p62) 1:100 (mouse monoclonal SC-48402, Santa Cruz) and anti-transforming growth factor β 3 (TGFB3) 1:25 (mouse monoclonal SC-166861, Santa Cruz). .. Antibody binding was detected using a Vectastain Elite ABC kit (Vector Laboratories, Burlingame, CA, USA) and 3,3′-diaminobenzidine as a chromogen.

    Binding Assay:

    Article Title: Sexual dimorphism in liver cell cycle and senescence signaling pathways in young and old rats
    Article Snippet: .. Primary antibodies used for IHC analysis were: for cell cycle, anti-cyclin-dependent kinase 2 (CDK2) 1:50 (mouse monoclonal SC-6248, Santa Cruz Biotechnology, Dallas, TX, USA), cyclin-dependent kinase 4 (CDK4) 1:400 (mouse monoclonal SC-23896, Santa Cruz), cyclin D1 (CycD, CCND1) 1:50 (mouse monoclonal SC-8396, Santa Cruz), anti-proliferating cell nuclear antigen (PCNA) 1:50 (mouse monoclonal SC-56, Santa Cruz), cyclin-dependent kinase inhibitor 1A (CDKN1A, p21) 1:50 (mouse monoclonal SC-6246, Santa Cruz) and cyclin-dependent kinase inhibitor 2B (CDKN2B, p15) 1:50 (mouse monoclonal SC-377412, Santa Cruz); for cellular senescence, anti-calmodulin 2 (CALM2) 1:50 (mouse monoclonal SC-137079, Santa Cruz), anti-insulin like growth factor binding protein 3 (IGFBP3)1:50 (mouse monoclonal SC-365936, Santa Cruz), anti-mechanistic target of rapamycin (mTOR) 1:50 (mouse monoclonal SC-517464, Santa Cruz), sequestosome 1 (SQSTM1, p62) 1:100 (mouse monoclonal SC-48402, Santa Cruz) and anti-transforming growth factor β 3 (TGFB3) 1:25 (mouse monoclonal SC-166861, Santa Cruz). .. Antibody binding was detected using a Vectastain Elite ABC kit (Vector Laboratories, Burlingame, CA, USA) and 3,3′-diaminobenzidine as a chromogen.



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    Image Search Results


    Inhibiting podocytes MDM2 expression alleviates renal pathological damage, and reduces podocytes dedifferentiation levels. (A) Representative images showing PAS and Masson staining (scale bar = 20 μm) in control, STZ, and shMDM2‐injected STZ groups. (B) IF staining of Ki‐67 (red), Cyclin B1 (green), Podocin (yellow), and MDM2 (pink), counterstained with DAPI (blue) in control, STZ and STZ injected with shMDM2 groups. (C) Protein levels of p21, Cyclin B1 and Podocin in control, STZ and STZ injected with shMDM2 groups, analysed by western blot. Data represent mean ± SD of three independent experiments.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: The Impact of METTL3 on MDM2 Promotes Podocytes Injury During Diabetic Kidney Disease

    doi: 10.1111/jcmm.70627

    Figure Lengend Snippet: Inhibiting podocytes MDM2 expression alleviates renal pathological damage, and reduces podocytes dedifferentiation levels. (A) Representative images showing PAS and Masson staining (scale bar = 20 μm) in control, STZ, and shMDM2‐injected STZ groups. (B) IF staining of Ki‐67 (red), Cyclin B1 (green), Podocin (yellow), and MDM2 (pink), counterstained with DAPI (blue) in control, STZ and STZ injected with shMDM2 groups. (C) Protein levels of p21, Cyclin B1 and Podocin in control, STZ and STZ injected with shMDM2 groups, analysed by western blot. Data represent mean ± SD of three independent experiments.

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    Techniques: Expressing, Staining, Control, Injection, Western Blot